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GrainGenes Reference Report: TAG-87-531

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Reference	TAG-87-531
Title	The genetic characterization of novel multi-addition doubled haploid lines derived from triticale X wheat hybrids
Journal	Theoretical and Applied Genetics
Year	1993
Volume	87
Pages	531-536
Author	Wang G [ Show all 6 ]
Abstract	Two novel 46-chromosome doubled haploid lines, W66 and M17, derived from separate hexaploid triticale X bread wheat crosses, were characterised using cytological and biochemical markers Both lines were shown to be relatively stable cytologically, over 11 and 8 generations of selfing, respectively By examining mitotic and meiotic chromosomes, the stabilities of the two lines were shown to be similar with frequencies of 2n = 46 in 74.2-85.5% of cells However, over selfed generations, the rye chromosomes were shown to have lost some of their heterochromatin, which made it difficult to establish their continued presence using cytological techniques, such as C-banding alone. Cytological evidence from pairing studies, C-banding, and fluorescence in-situ hybridization, showed that both M17 and W66 are wheat/rye multi-addition lines with rye chromosome constitutions of 1R + 6R, and 1R + 4R, respectively. These conclusions were confirmed by isozyme and storage-protein analysis.
Keyword	[ Hide all but 1 of 17 ] addition-lines c-bands chromosome-addition chromosome-analysis chromosome-banding chromosome-number chromosome-pairing cytology dna hybridization haploid heterochromatin hybridization isoenzyme plant proteins seed triticale triticum aestivum

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