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GrainGenes Reference Report: TAG-89-599

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Reference
TAG-89-599
Title
Hybrids and backcross progenies between wheat (Triticum aestivum L.) and apomictic Australian wheatgrass [Elymus rectisetus (Nees in Lehm.) A. Love & Connor]: karyotypic and genomic analyses
Journal
Theoretical and Applied Genetics
Year
1994
Volume
89
Pages
599-605
Author
Liu Z (2)
Wang R
Carman J
Abstract
Wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) florets were emasculated and pollinated using two apomictic wheatgrass [Elymus rectisetus (Nees in Lehm. A Love & Connor, 2n = 6x = 42, SSYYWW] accessions, one of which produces 2n pollen A 2n = 42 (BII) hybrid and four 2n = 63 (BIII) hybrids were obtained The spike morphology of the BII hybrid was intermediate to that of its parents The pollen mother cells (PMCs) of this hybrid contained on average 38.36 I and 1.62 II, which was consistent with its disparate genome composition (ABDSYW). Its pollen failed to stain and no BC1 progeny was obtained. The BIII hybrids (reduced egg fertilized with unreduced sperm) were grasslike and had a full complement of E. rectisetus chromosomes, the synapsis of which was slightly impaired by wheat haplome and/or cytoplasm. Their PMCs contained on average 16.30 II, 25.72 I, and 1.54 multivalents (III plus IV). Pollen stainability in these hybrids was low (< 1%). and when they were used as females, one 54- and 60-chromosome BC1 were obtained. A mean of 13.25 II was observed in PMCs of the 54-chromosome BC, and pollen stainability was 10%. Pollen stainability in the 60-chromosome BC1 was only 5%. The use of 2n-pollen-producing E. rectisetus accession accelerated hybrid and BC1 formation and may accelerate the ultimate transfer of apomixis to wheat.
Keyword
[ Hide all but 1 of 17 ]
apomixis
backcross
backcrossing
chromosome-number
chromosome-pairing
cytogenetics
elymus
genome-analysis
hybrids
intergeneric-hybridization
karyotype
male-fertility
meiosis
pollen
staining
triticum aestivum
wide-hybridization

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