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GrainGenes Reference Report: TAG-89-676

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Reference	TAG-89-676
Title	Genetic diversity among wild and cultivated barley as revealed by RFLP
Journal	Theoretical and Applied Genetics
Year	1994
Volume	89
Pages	676-681
Author	Peterson L Ostergard H Giese H
Abstract	Genetic variability of cultivated and wild barley, Hordeum vulgare ssp vulgare and spontaneum, respectively, was assessed by RFLP analysis The material consisted of 13 European varieties, single-plant offspring lines of eight land races from Ethiopia and Nepal, and five accessions of ssp spontaneum from Israel, Iran and Turkey. Seventeen out of twenty-one studied cDNA and gDNA probes distributed across all seven barley chromosomes revealed polymorphism when DNA was digested with one of four restriction enzymes. A tree based on genetic distances using frequencies of RFLP banding patterns was estimated and the barley lines clustered into five groups reflecting geographical origin. The geographical groups of land-race lines showed less intragroup variation than the geographical groups of spontaneum lines. The group of European varieties, representing large variation in agronomic traits, showed an intermediate level. The proportion of gene diversity residing among geographical groups (FST) varied from 0.19 to 0.94 (average 0.54) per RFLP pattern, indicating large diversification between geographical groups.
Keyword	[ Hide all but 1 of 18 ] dna probes ethiopia europe genetic distance genetic variation geographical-variation hordeum spontaneum hordeum vulgare hordeum vulgare subsp. iran israel landrace nepal restriction fragment length polymorphism spontaneum turkey varieties vulgare

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