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GrainGenes Reference Report: TAG-89-927

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Reference	TAG-89-927
Title	Identification of RFLP markers linked to the cereal cyst nematode resistance gene (Cre) in wheat
Journal	Theoretical and Applied Genetics
Year	1994
Volume	89
Pages	927-930
Author	Williams KJ Fisher JM Langridge P
Abstract	The cereal cyst nematode (CCN) (Heterodera avenae Woll.) is an economically damaging pest of wheat in many of the worlds cereal growing areas. The development of CCN-resistant cultivars may be accelerated by the use of molecular markers. The Cre gene of the wheat line 'AUS10894' confers resistance to CCN. Using a pair of near-isogenic lines (NILs) that should differ only in a small chromosome segment containing the Cre locus, we screened 58 group-2 probes and found two (Tag605 and CDO588) that detect polymorphism between the NILs. Nulli-tetrasomic and ditelosomic lines confirmed that the restriction fragment length polymorphism (RFLP) markers identified were derived from the long arm of wheat chromosome 2. Crosses between 'AUS10894' and 'Spear' and the NIL 'AP' and its recurrent parent 'Prins' were used to produce F2 populations that gave the expected 3:1 segregation ratio for the resistance gene. Linkage analysis identified two RFLP markers flanking the resistance gene. Xglk605 and Xcdo588 mapped 7.3 cM (LOD=6.0) and 8.4 cM (LOD=6.7), respectively, from the Cre locus
Keyword	[ Hide all but 1 of 33 ] cereal chromosome chromosome maps development ditelosomic dominance fragment length polymorphism gene genetic mapping genetic markers heterodera avenae hordeum vulgare its linkage linkage analysis linkage groups locus long arm marker molecular marker near isogenic lines near-isogenic lines nematode resistance pest resistance polymorphism population probes resistance resistance gene rflp rflp markers segregation woll

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