Query (optional)   in Class  

GrainGenes Reference Report: TAG-89-982

[Submit comment/correction]

Reference
TAG-89-982
Title
Study of androgenetic performance and molecular characterization of a set of wheat-rye addition lines
Journal
Theoretical and Applied Genetics
Year
1994
Volume
89
Pages
982-990
Author
Martinez I
Bernard M
Nicolas P
Bernard S
Abstract
Rye chromosomes of wheat-rye addition lines were successfully identified by means of an RFLP analysis with 30 probes. Our results are in agreement with previous cytological data concerning the identity of lines F (+1R) D (+2R), C (+3R), A (+4R), E (+5R) and B (+7R). Two categories of chromosomal rearrangements have been distinguished, namely: (1) deletions: the current line D possesses a chromosome 2R deleted on its short arm and the line G a chromosome 3R deleted on its long arm; we have also noticed a deletion on the long arm of wheat chromosome 1A in line F61; and (2) evolutionary reciprocal translocations in rye relative to wheat which have been previously mentioned in the literature. The anther culture response of the different lines was studied. A significant difference between 'FEC 28' and the addition lines was observed for embryo production and plant regeneration. It appears that genes located on 'S 10' chromosome arm 3RL and on 'FEC 28' chromosome arm 1AL increase embryo frequency whereas gene(s) located on 'S 10' chromosome 5R reduce(s) it. Plant regeneration results suggest that genes increasing regeneration ability and green-plant frequency are located on 'S 10' chromosome 4R. The long arm of chromosome 1A seems to be involved positively in green-plant regeneration whereas chromosomes 1R and 3R limit plant regeneration
Keyword
[ Hide all but 1 of 32 ]
addition lines
androgenesis
anther culture
chromosome
chromosome translocation
culture
deletion
dna hybridization
dna probes
embryo
gene
gene location
genetic markers
interspecific hybridization
its
line differences
long arm
molecular characterization
performance
plant embryos
plant regeneration
probes
rearrangements
regeneration
regenerative ability
rflp
rflp analysis
rye
secale cereale
translocation
triticum aestivum
triticum x secale

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.