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GrainGenes Reference Report: TAG-89-1014

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Reference	TAG-89-1014
Title	CMS in rye: comparative RFLP and transcript analyses of mitochondria from fertile and male-sterile plants
Journal	Theoretical and Applied Genetics
Year	1994
Volume	89
Pages	1014-1018
Author	Dohmen G Hessberg H Geiger HH Tudzynski P
Abstract	The mitochondrial (mt) genomes of rye (Secale cereale L.) lines with 'normal' and cytoplasmic male sterility (CMS) inducing 'Pampa' cytoplasm were compared by detailed restriction fragment length polymorphism (RFLP) and Northern analyses. RFLP analyses using several heterologous mt genes as probes revealed considerable differences in the overall structure of the two mt genomes. With cob and atpA, the data indicate intragenic re-combination and/or different copy numbers of these genes in the two cytoplasms. In spite of this heterogeneity at DNA level, the transcriptional patterns of nine out of ten mitochondrial genes analysed are unaffected. The exception is in the 'Pampa' cytoplasm which contains an additional cob-homologous transcript. Since this transcript is strongly reduced in the presence of restorer genes, it might causally be correlated to the CMS phenotype
Keyword	[ Hide all but 1 of 25 ] copy number cytoplasm cytoplasmic male sterility dna dna probes fertile fragment length polymorphism gene gene expression genome heterogeneity intragenic recombination male fertility male sterility messenger rna mitochondria mitochondrial dna polymorphism probes recombination restorer gene rflp rye secale cereale transcription

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