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GrainGenes Reference Report: TAG-90-595

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Reference	TAG-90-595
Title	Visualization of Secale cereale DNA in wheat germ plasm by fluorescent in situ hybridization
Journal	Theoretical and Applied Genetics
Year	1995
Volume	90
Pages	595-600
Author	Islam-Faridi M Mujeeb-Kazi A
Abstract	Homozygous wheat/rye (1BL/1RS or 1AS/1RL) translocation lines have significantly contributed to wheat production, and several other wheat/rye translocation lines show a potential promise against biotic and abiotic stresses Detecting the presence of rye at the chromosome level is feasibie by C-banding and isozyme protocols, but the diagnostic strength of genomic in situ hybridization for eventually analyzing smaller DNA introgressions has greater significance As a first step we have applied the genomic in situ hybridization technique to detect rye chromosomes in a wheat background using germ plasm of agricultural significance By this method rye contributions to the translocations 1BL/1RS, 1AL/1RS, SAS/SRL and 6BS/6RL could be identified Differential labelling has further enabled the detection of rye and Thinopyrum bessarabicum chromosomes in a trigeneric hybrid of Triticum aestivum/Th bessarabicum//Secale cereale.
Keyword	[ Hide all but 1 of 19 ] biotin chromosome chromosome-addition chromosome-translocation detection dna hybridization dna probes fluorescence germplasm hybrids intergeneric-hybridization labeling secale cereale thinopyrum thinopyrum bessaraabicum translocation-lines triticale triticum aestivum triticum turgidum

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