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GrainGenes Reference Report: TAG-90-740

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Reference	TAG-90-740
Title	Generation of PCR-based markers for the detection of rye chromatin in a wheat background
Journal	Theoretical and Applied Genetics
Year	1995
Volume	90
Pages	740-745
Author	Koebner R
Abstract	Oligonucleotide primers were developed to detect the presence of four rye sequences using a PCR assay These assays give a rye-specific signal from wheat DNA template which contains various rye chromosomes or chromosome segments The sequences identified were associated with the nucleolar organiser region, the 5S-Rrna-R1 locus, the telomere, and a widely dispersed, rye-specific repetitive element Ris-1 The primers amplified from the well-established loci Nor-R1 and 5S-Rrna-R1 on rye chromosome arm 1RS, and also located a 5s-Rrna locus on chromosome 3R The telomere-associated sequence was present on every rye chromosome, and was also present, at a low copy number, in both wheat and barley These assays will be particularly useful for introgression programmes aimed at reducing the rye content of the 1BL.1RS wheat-rye translocation. When multiplexed, the primers will enable a rapid, simultaneous assay for a number of distinct rye loci, which can be derived from a small portion of mature endosperm tissue.
Keyword	[ Hide all but 1 of 13 ] chromatin chromosome dna genetic markers loci nucleolus-organizer nucleotide sequence polymerase chain reaction repetitive dna ribosomal dna secale cereale telomeric-dna triticum aestivum

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