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GrainGenes Reference Report: TAG-91-1288

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Reference	TAG-91-1288
Title	The transfer of a powdery mildew resistance gene from Hordeum bulbosum L. to barley (H. vulgare L.) chromosome 2 (2I)
Journal	Theoretical and Applied Genetics
Year	1995
Volume	91
Pages	1288-1292
Author	Pickering R Hill A Michel M Timmerman-Vaughan G
Abstract	Hordeum bulbosum L is a source of disease resistance genes that would be worthwhile transferring to barley (H vulgare L. To achieve this objective, selfed seed from a tetraploid H vulgare X H bulbosum hybrid was irradiated. Subsequently, a powdery mildew-resistant selection of barley phenotype (81882/83) was identified among field-grown progeny. Using molecular analyses, we have established that the H. bulbosum DNA containing the powdery mildew resistance gene had been introgressed into 81882/83 and is located on chromosome 2 (2I). Resistant plants have been backcrossed to barley to remove the adverse effects of a linked factor conditioning triploid seed formation, but there remains an association between powdery mildew resistance and non-pathogenic necrotic leaf blotching. The dominant resistance gene is allelic to a gene transferred from H. bulbosum by co-workers in Germany, but non-allelic to all other known powdery mildew resistance genes in barley. We propose Mlhb as a gene symbol for this resistance.
Keyword	[ Hide all but 1 of 14 ] chromosome disease resistance dominance erysiphe graminis-f gene-location genes hordeum bulbosum hordeum vulgare inheritance interspecific-hybridization introgression irradiation mlhb gene plant breeding

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