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GrainGenes Reference Report: TAG-99-487

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Reference	TAG-99-487
Title	Single-stranded DNA in the genetic transformation of wheat (Triticum aestivum L.): transformation frequency and integration pattern.
Journal	Theoretical and Applied Genetics
Year	1999
Volume	99
Pages	487-495
Author	Uze M Potrykus I Sautter C
Abstract	Summary: Two non-linked marker genes (gus and bar) were co-introduced by microprojectile bombardment into wheat cells. Four different DNA structures were compared with respect to ability to integrate into the wheat genome: circular or linear (1) DNA as a single- or double-stranded plasmid (ss and ds, respectively). In eight independent experiments, linearized DNA integrated in the ds or ss form with a high efficiency of up to 14% for l-ssDNA. Molecular analyses by Southern blotting showed that all DNA forms gave a similar complicated integration pattern of the bar gene
External Databases	http://link.springer.de/link/service/journals/00122/bibs/9099003/90990487.htm
Keyword	[ Hide all but 1 of 21 ] acyltransferases beta glucuronidase biolistics dna dna conformation enzyme activity genetic transformation glufosinate herbicide resistance histoenzymology integration marker genes plant embryos plasmid vectors recombination reporter gene single-stranded dna southern blotting transformation triticum triticum aestivum

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