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GrainGenes Reference Report: TAG-99-727

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Reference	TAG-99-727
Title	RFLP mapping of BaYMV resistance gene rym3 in barley (Hordeum vulgare).
Journal	Theoretical and Applied Genetics
Year	1999
Volume	99
Pages	727-732
Author	Saeki K [ Show all 6 ]
Abstract	Summary: The rym3 (formerly designated ym3) gene conferring resistance to barley yellow mosaic virus (BaYMV) is effective against all strains of the virus but up to now has not been mapped to any chromosome. We performed a linkage analysis, using DNA extracted from individually harvested mature leaves of 153 F(2) plants derived from a cross between BaYMV-resistant cv 'Ishuku Shirazu' carrying rym3 and susceptible cv 'Ko A'. Additionally, the F(3) lines derived from F(2) plants were grown in the BaYMV-infested field and examined for their reaction to BaYMV. Our results indicated that rym3 is located on the short arm of chromosome 5H and flanked by RFLP markers MWG28 and ABG705A at distances of 7.2 and 11.7 cM, respectively. The chromosomal configuration estimated by DNA markers around rym3 and the utilization of these molecular markers for pyramiding with the BaYMV resistance genes in barley breeding programs are discussed
External Databases	http://link.springer.de/link/service/journals/00122/bibs/9099003/90990727.htm
Keyword	[ Hide all but 1 of 15 ] barley barley yellow mosaic virus breeding programs chromosome maps disease resistance dna gene location genetic mapping genetic markers hordeum vulgare linkage linkage groups resistance rflp segregation

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