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GrainGenes Reference Report: TAG-100-271

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Reference	TAG-100-271
Title	A spelt-specific gamma-gliadin gene: discovery and detection
Journal	Theoretical and Applied Genetics
Year	2000
Volume	100
Pages	271-279
Author	Buren Mv Luthy J Hubner P
Abstract	Summary: Polymerase chain reaction (PCR) primers GAG5 and GAG6 were designed based on published gamma-gliadin gene sequences and applied to 35 cultivars of closely related spelt (Triticum spelta L.) and hexaploid wheat (T. aestivum L.). Eight tetraploid durum wheat (T. durum Desf.) cultivars were included in the analysis. The obtained PCR products originated from two gamma-gliadin genes which were mapped to homeologous chromosomes 1B and 1D and termed GAG56B and GAG56D, respectively. Two alleles of GAG56D differing in a 9-bp deletion/duplication and single nucleotide polymorphism were found. The 18 spelts tested and wheat cultivar 'Chinese Spring' were discovered to carry a previously unknown gamma-gliadin gene, while 16 wheat cultivars possessed its longer, already published allele. Two PCR-based detection systems for the diagnostic alleles were developed and applied. The occurrence of two alleles of GAG56B among the investigated durum wheats correlated with their expression of gluten quality markers gamma-gliadins 42 or 45
Keyword	[ Hide all but 1 of 15 ] alleles amino acid sequence detection dna gene location genetic polymorphism gliadin gluten polymerase chain reaction polymorphism quality triticum triticum aestivum triticum durum triticum spelta

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