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GrainGenes Reference Report: TAG-100-396

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Reference	TAG-100-396
Title	A 1B-coded low-molecular weight glutenin subunit associated with quality in durum wheats shows strong similarity to a subunit present in some bread wheat cultivars
Journal	Theoretical and Applied Genetics
Year	2000
Volume	100
Pages	396-400
Author	Masci S D'Ovidio R Lafiandra D Kasarda DD
Abstract	Summary: Good quality durum wheats usually present the LMW-2 type of SDS-PAGE pattern, whereas the LMW-1 type of pattern is usually associated with poor quality durum wheats. The two patterns are distinguished mainly by the presence of a strongly expressed protein band with molecular weight around 42,000 (42 K subunit) in the LMW-2-type pattern; this subunit is absent in the LMW-1-type pattern. Here we show that this particular low-molecular-weight glutenin subunit has strong similarity to a subunit present in some bread wheat cultivars. This correspondence has been demonstrated through SDS-PAGE, PCR analysis of the corresponding genes, a comparison of the deduced amino acid sequences, and RP-HPLC. This last approach showed a slight difference in retention time between the 42 K protein of bread and durum wheats that might be attributed to the eight amino acid differences found between the deduced amino acid sequences of the two corresponding genes
External Databases	http://link.springer.de/link/service/journals/00122/bibs/0100003/01000396.htm
Keyword	[ Hide all but 1 of 37 ] allelic variation amino acid amino acid sequence animal bread bread wheat dough properties durum durum wheat gene glutenin glutenin subunit hplc hydrophobicity lmw lmw glutenin genes lmw glutenin subunits low-molecular weight glutenin genes low-molecular weight glutenin subunits low-molecular-weight glutenin genes low-molecular-weight glutenin subunits molecular weight page pcr polyacrylamide-gel electrophoresis polymerase chain reaction prediction progeny quality rp-hplc sds page sequence species differences triticum durum weight wheat cultivar wheat quality

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