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GrainGenes Reference Report: TAG-102-477

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Reference	TAG-102-477
Title	Molecular mapping of a fertility restoration locus (Rfm1) for cytoplasmic male sterility in barley (Hordeum vulgare L.)
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	477-482
Author	Matsui K Mano Y Taketa S Kawada N Komatsuda T
Abstract	Summary: The Rfm1a gene restores the fertility of msm1 cytoplasmic male-sterile lines in barley. We identified three RAPD markers linked to the Rfm1 locus (CMNB-07/800, OPI-18/900, and OPT-02/700) using isogenic lines and segregating BC1F1 and F2 populations. Using a previously developed linkage map of barley, we located CMNB-07/800 and OPT-02/700 beside MWG2218 on chromosome 6HS. The linkage between MWG2218 and the Rfm1 locus was demonstrated using the segregating BC1F1 and F2 populations. To confirm the chromosomal locations of these markers, we converted them to STSs and tested against two sets of wheat-barley chromosome addition lines. These STS markers, CMNB-07/800, OPT-02/700, and MWG2218, were amplified only in the addition lines possessing the chromosome 6H, thereby providing additional evidence the Rfm1 locus is located on chromosome 6H. Homoeologous relationships among fertility restoration genes in Triticeae are discussed
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1102004/11020477.htm
Keyword	[ Hide all but 1 of 39 ] addition lines barley chromosomal location chromosome chromosome addition chromosome addition lines corn cytoplasmic male sterility cytoplasmic male sterility (cms) f-2 population fertility fertility restoration gene genome homoeologous relationships hordeum vulgare isogenic lines linkage linkage map locus maize male sterility map mapping marker molecular mapping population randomly amplified polymorphic dna (rapd) sequence tagged site (sts) rapd rapd markers restoration restorer gene rf2 nuclear restorer rflp rye sterility sts sts marker triticeae

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