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GrainGenes Reference Report: TAG-102-651

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Reference	TAG-102-651
Title	Molecular cytogenetic characterization of Roegneria ciliaris chromosome additions in common wheat
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	651-657
Author	Wang XE [ Show all 7 ]
Abstract	Summary: The development of alien addition lines is important both for transferring useful genes from related species into common wheat and for studying the relationship between alien chromosomes and those of wheat. Roegneria ciliaris (2n=4x=28, ScScYcYc) is reported to be a potential source of resistance to wheat scab, which may be useful in wheat improvement. The amphiploid common wheat-R. ciliaris and BC1F7 or BC2F6 derivatives were screened by C-banding, genomic in situ hybridization (GISH), fluorescent in situ hybridization (FISH) and restriction fragment length polymorphism (RFLP) for the presence of R. ciliaris chromatin introgressed into wheat. Six lines were identified as disomic chromosome additions (DA), one as a ditelosomic addition (Dt), two as double disomic additions (dDA) and one as a monosomic chromosome addition (MA). RFLP analysis using wheat homoeologous group-specific clones indicated that the R. ciliaris chromosomes involved in these lines belong to groups 1, 2, 3, 5 and 7. The genomic affinities of the added R. ciliaris chromosomes were determined by FISH analysis using the repetitive sequence pCbTaq4.14 as a probe. These data suggest that the R. ciliaris chromosomes in five lines belong to the Sc genome. Based on the molecular cytogenetic data, the lines are designated as DA2Sc#1, Dt2Sc#1L, DA3Sc#1, dDA1Sc#2+5Yc#1, DA5Yc#1, DA7Sc#1, DA7Yc#1 and MA?Yc#1. Based on the present and previous work, 8 of the 14 chromosomes of R. ciliaris have been transferred into wheat
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1102005/11020651.htm
Keyword	[ Hide all but 1 of 47 ] addition lines afa-family affinities agropyron bread wheat c banding characterization chromatin chromosome chromosome addition clone common wheat derivatives development ditelosomic elymus trachycaulus fish fluorescent in situ hybridization fragment length polymorphism gene genetic linkage map genome genomic affinities genomic in situ hybridization gish homoeology hybridization improvement in situ in situ hybridization length polymorphism line molecular cytogenetics plant science polymorphism progenitor repetitive dna repetitive sequence resistance rflp rflp analysis roegneria ciliaris scab sequence situ hybridization triticum aestivum wheat scab

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