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GrainGenes Reference Report: TAG-103-601

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Reference	TAG-103-601
Title	Genetic analysis of kernel hardness in bread wheat using PCR-based markers
Journal	Theoretical and Applied Genetics
Year	2001
Volume	103
Pages	601-606
Author	Galande AA [ Show all 10 ]
Abstract	Summary: In wheat, kernel hardness is a complex genetic trait involving various directly and indirectly contributing components such as kernel hardness per se, protein content, hectolitre weight and 1,000-kernel weight. In an attempt to identify DNA markers associated with this trait, 100 recombinant inbred lines (RILs) derived from a cross between a hard grain land-race, NP4, and a soft grain variety, HB 208, were screened with 100 ISSR and 360 RAPD primers. Eighteen markers were assigned to seven linkage groups covering 223.6 cM whereas 11 markers remained unlinked. A multiple-marker model explained the percentage of phenotypic variation for kernel hardness as 20.6%, whereas that for protein content, hectolitre weight and 1,000-kernel weight was 18.8%, 13.5% and 12.1%, respectively. Our results indicate that phenotypic expression of kernel hardness is controlled by many QTLs and is interdependent on various related traits
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1103004/11030601.htm
Keyword	[ Hide all but 1 of 36 ] baking quality bread bread wheat complexes component cross dna dna marker friabilin genetic analysis grain hardness hardness inbred lines issr kernel kernel hardness kernel hardness (kh) landrace linkage linkage group marker model pcr-based marker phenotypic variation primer protein protein content qtl quantitative trait loci (qtl) rapd recombinant inbred line recombinant inbred lines (rils) rils soft softness trait

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