GrainGenes Reference Report: TAG-103-696
Reference
TAG-103-696
Title
Introgression of chromosomes of Festuca arundinacea var. glaucescens into Lolium multiflorum revealed by genomic in situ hybridisation (GISH)
Journal
Theoretical and Applied Genetics
Year
2001
Volume
103
Pages
696-701
Author
Morgan WG King IP Koch S Harper JA Thomas HM
Abstract
Summary: An F1 hybrid (n=4x=28) between the tetraploid species Festuca arundinacea var. glaucescens (GGG'G') and a synthetic tetraploid Lolium multiflorum (LmLmLmLm) was backcrossed to diploid L. multiflorum to produce triploid (2n=3x=21) BC1 hybrids (LmLmG). At metaphase I of meiosis the triploids had a preponderance of ring bivalents and univalents with some linear and frying-pan trivalents. Genomic in situ hybridisation (GISH) differentiated the Festuca chromosomes from Lolium and revealed that the bivalents were exclusively between Lolium homologues, while the univalents were Festuca. Despite the limited amount of homoeologous chiasmata pairing in the triploids, some recombinant chromosomes were recovered in the second backcross when the hybrids were further crossed to diploid L. multiflorum. The progeny from the second backcross was predominantly diploid. Genotypes with recombinant chromosomes and chromosome additions involving an extra Festuca chromosome were identified using GISH. Changes in plant phenotype were related to the presence of Festuca chromatin
External Databases
http://link.springer-ny.com/link/service/journals/00122/bibs/1103005/11030696.htm
Keyword
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-103-696
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||