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GrainGenes Reference Report: TAG-104-518

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Reference	TAG-104-518
Title	Cre/lox site-specific recombination controls the excision of a transgene from the rice genome
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	518-525
Author	Hoa TTC Bong BB Huq E Hodges TK
Abstract	Summary: The Cre/lox site-specific recombination controls the excision of a target DNA segment by recombination between two lox sites flanking it, mediated by the Cre recombinase. We have studied the functional expression of the Cre/lox system to excise a transgene from the rice genome. We developed transgenic plants carrying the target gene, hygromycin phosphotransferase (hpt) flanked by two lox sites and transgenic plants harboring the Cre gene. Each lox plant was crossed with each Cre plant reciprocally. In the Cre/lox hybrid plants, the Cre recombinase mediates recombination between two lox sites, resulting in excision of the hpt gene. The recombination event could be detected because it places the CaMV 35S promoter of the hpt gene adjacent to a promoterless gusA gene; as a result the gusA gene is activated and its expression could be visualized. In 73 Cre/lox hybrid plants from various crosses of T0 transgenic plants, 19 expressed GUS, and in 132 Cre/lox hybrid plants from crosses of T2 transgenic plants, 77 showed GUS expression. Molecular data proved the excision event occurred in all the GUS(+) plants. Recombination occurred with high efficiency at the early germinal stage, or randomly during somatic development stages
External Databases	http://link.springer.de/link/service/journals/00122/papers/2104004/21040518.pdf
Keyword	[ Hide all but 1 of 41 ] 35s promoter arabidopsis thaliana camv 35s camv 35s promoter chromosome cre cre/lox recombination system cross dna excision functional expression gene genome gus gus expression gusa gusa gene high-efficiency hpt hpt gene hybrid hybrid plants hygromycin integration mediated recombination oryza sativa plant genome promoter recombination rice rice genome segment site site-specific recombination system target transformation transgene transgenic transgenic plant transient expression

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