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GrainGenes Reference Report: TAG-104-845

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Reference	TAG-104-845
Title	The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	845-851
Author	Fernandez ME Figueiras AM Benito C
Abstract	Summary: The potential of bulk analyses of RAPD and ISSR-PCR markers for fingerprinting purposes was evaluated using ten RAPD and ten ISSR primers. The phylogenetic relationships of 16 barley cultivars from different countries, and all having a known pedigree, were analysed using 353 PCR markers (125 RAPDs and 228 ISSRs). The band profiles generated were reproducible in spite of the different DNA extractions, PCR techniques, electrophoretic methods and gel scorings used. The RAPD primer S10 and four ISSR primers (811, 820, 835 and 881) were both able to distinguish all cultivars. A strong and quite linear relationship was observed between Resolving Power (Rp) of a primer and its ability to distinguish genotypes. The dendrograms obtained using these two molecular markers are in agreement with their known origin, showing clusters that separate very well the spring/winter and six-rows/two-rows cultivars. Thus, bulk analyses of RAPD and ISSR PCR markers provides a quick, reliable and highly informative system for DNA fingerprinting and also permit to establish genetic relationships which agree with, by other means, known origin of the cultivars
External Databases	http://link.springer.de/link/service/journals/00122/contents/01/00848/paper/s00122-001-0848-2.pdf
Keyword	[ Hide all but 1 of 40 ] abilities accessions amplification barley barley cultivars bulked analyses cluster collection distinguish diversity dna dna extraction dna fingerprinting dna polymorphism extraction fingerprinting genetic diversity genetic relationship genotype genotype identification germplasm issr issr-pcr issrs method molecular marker origin pcr pcr markers pedigree phylogenetic relationship polymerase polymorphism population rapd rapd markers rapds relationships rflp sequence repeat markers

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