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GrainGenes Reference Report: TAG-104-1071

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Reference	TAG-104-1071
Title	Molecular cloning of the wheat CK2 alpha gene and detection of its linkage with Vrn-A1 on chromosome 5A
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	1071-1077
Author	Kato K Kidou S Miura H Sawada S
Abstract	Summary: The casein kinase CK2 is one of the major multifunctional protein kinases in cells that is expressed ubiquitously and is essential for survival. The alpha-subunit of CK2 is thought to be involved in light-regulated gene expression and rhythmic expression of genes by circadian rhythm in plants. The rice chromosome-3 region containing the photoperiod-response Hd6 gene, an orthologue of the CK2alpha genes of Arabidopsis and maize, is in synteny with the wheat chromosome-5A Vrn-A1 region. This evidence proposes two possibilities, first the wheat Vrn-A1 is an orthologue of the rice CK2alpha, and second the wheat CK2alpha which has not yet been identified is located independently but tightly linked to Vrn-A1. To clarify whether the wheat CK2alpha gene is conserved in the Vrn-A1 region and to elucidate the above two possibilities, we attempted to isolate this gene from the wheat cDNA library and to map it on the chromosome-5A region that is syntenous to the rice Hd6 region. The isolated cDNA clone showed an extremely high homology with the Arabidopsis CK2alpha gene. Using this clone as a probe genomic Southern-blot analyses of the aneuploid lines available in Chinese Spring assigned the wheat homologue of CK2alpha to the long arm of chromosome 5A. Furthermore, a linkage analysis using an F2 population having recombination in the Vrn-A1 region revealed that the wheat CK2alpha, designated as tck2a, is tightly linked to Vrn-A1 by 1.1 cM
External Databases	http://link.springer.de/link/service/journals/00122/contents/01/00805/paper/s00122-001-0805-0.pdf
Keyword	[ Hide all but 1 of 51 ] 5a aegilops speltoides alpha alpha-subunit aneuploid aneuploid lines arabidopsis casein casein kinase 2 alpha casein kinase-ii cdna cdna clone cdna library cell chinese chinese spring chromosome chromosome 5a circadian rhythm clone cloning detection ear-emergence time f-2 population gene homology isolate kinase kinase ck2 linkage linkage analysis long arm maize major genes map molecular cloning photoperiod response plant height population probe protein protein kinase quantitative trait locus recombination region rice spring survival synteny vernalization vrn-a1

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