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GrainGenes Reference Report: TAG-105-660

Reference	TAG-105-660
Title	Integration of microsatellite markers into the translocation-based physical RFLP map of barley chromosome 3H
Journal	Theoretical and Applied Genetics
Year	2002
Volume	105
Pages	660-665
Author	Kunzel G Waugh R
Abstract	Summary: PCR with the DNA of translocation chromosomes and marker-specific primers has been used to merge genetically mapped microsatellite (MS) markers into the physically integrated restriction fragment length polymorphism (RFLP) map of barley chromosome 3H. It was shown that the pronounced clustering of MS markers around the centromeric region within the genetic map of this chromosome results from suppressed recombination. This yielded a refinement of the physically integrated RFLP map of chromosome 3H by subdivision of translocation breakpoints (TBs) that were previously not separated by markers. The physical distribution of MS markers within most of the subchromosomal regions corresponded well with that of the RFLP markers, indicating that both types of markers are similarly valuable for a wide range of applications in barley genetics
External Databases	http://link.springer.de/link/service/journals/00122/contents/02/00913/paper/s00122-002-0913-5.pdf
Keyword	[ Hide all but 1 of 25 ] barley cultivated potato dna dna marker fragment length polymorphism genetic map hordeum vulgare linkage map localization map microisolated chromosomes microsatellite marker organization oryza sativa pcr physical mapping polymorphism reciprocal translocations recombination rflp rflp map simple sequence repeat sugar beet tomato genome translocation

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