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GrainGenes Reference Report: TAG-106-1059

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Reference	TAG-106-1059
Title	RAPD polymorphisms in spring wheat cultivars and lines with different level of Fusarium resistance
Journal	Theoretical and Applied Genetics
Year	2003
Volume	106
Pages	1059-1067
Author	Sun G Bond M Nass H Martin R Dong Z
Abstract	Summary: Random amplified polymorphic DNA (RAPD) markers have been used to characterize the genetic diversity among 35 spring wheat cultivars and lines with different levels of Fusarium resistance. The objectives of this study were to determine RAPD-based genetic similarity between accessions and to derive associations between Fusarium head blight (FHB) and RAPD markers. Two bulked DNA from either highly resistant lines or susceptible lines were used to screen polymorphic primers. Out of 160 screened primers, 17 primers generated reproducible and polymorphic fragments. Genetic similarity calculated from the RAPD data ranged from 0.64 to 0.98. A dendrogram was prepared on the basis of a similarity matrix using the UPGMA algorithm, which corresponded well with the results of principal component analysis and separated the 35 genotypes into two groups. Association analysis between RAPD markers and the FHB index detected three RAPD markers, H191000, F2500 and B12400, significantly associated with FHB-resistant genotypes. These results suggest that a collection of unrelated genotypes can be used to identify markers linked to an agronomically important quantitative trait like FHB. These markers will be useful for marker-assistant breeding and cloning
Keyword	[ Hide all but 1 of 44 ] accessions amplified polymorphic dna associations blight bread wheat breeding cloning cluster analysis collection combining ability diversity dna dna marker dna polymorphism fhb fragments fusarium fusarium head blight gene gene mapping genetic diversity head blight head blight resistance mapping matrix microsatellite marker objectives pedigree primer principal component analysis quantitative random amplified polymorphic dna rapd rapd marker rapd markers resistance resistant rflp scab resistance similarities spring spring wheat spring wheat cultivars trait

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