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GrainGenes Reference Report: TAG-107-503

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Reference	TAG-107-503
Title	Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread
Journal	Theoretical and Applied Genetics
Year	2003
Volume	107
Pages	503-508
Author	Buerstmayr H [ Show all 9 ]
Abstract	Fusarium head blight (FHB, scab) causes severe yield and quality losses, but the most serious concern is the mycotoxin contamination of cereal food and feed. The cultivation of resistant varieties may contribute to integrated control of this fungal disease. Breeding for FHB resistance by conventional selection is feasible, but tedious and expensive. The aim of this work was to detect QTLs for combined type I and type II resistance against FHB and estimate their effects in comparison to the QTLs identified previously for type II resistance. A population of 364, F1 derived doubled-haploid (DH) lines from the cross 'CM-82036' (resistant)/'Remus' (susceptible) was evaluated for components of FHB resistance during 2 years under field conditions. Plants were inoculated at anthesis with a conidial suspension of Fusarium graminearum or Fusarium culmorum. The crop was kept wet for 20 h after inoculation by mist-irrigation. Disease severity was assessed by visual scoring. Initial QTL analysis was performed on 239 randomly chosen DH lines and extended to 361 lines for putative QTL regions. Different marker types were applied, with an emphasis on PCR markers. Analysis of variance, as well as simple and composite interval mapping, revealed that two genomic regions were significantly associated with FHB resistance. The two QTLs on chromosomes 3B (Qfhs.ndsu-3BS) and 5A (Qfhs.ifa-5A) explained 29 and 20% of the phenotypic variance, respectively, for visual FHB severity. Qfhs.ndsu-3BS appeared to be associated mainly with resistance to fungal spread, and Qfhs.ifa-5A primarily with resistance to fungal penetration. Both QTL regions were tagged with flanking SSR markers. These results indicate that FHB resistance was under the control of two major QTLs operating together with unknown numbers of minor genes. Marker-assisted selection for these two major QTLs appears feasible and should accelerate the development of resistant and locally adapted wheat cultivars.
External Databases	Pubmed: 12768240 https://doi.org/10.1007/s00122-003-1272-6
QTL	Qfhs.ifa-5A
Keyword	[ Hide all but 1 of 57 ] analysis of variance blight bread wheat breeding composite interval mapping contamination control culmorum cultivation disease doubled haploid feed fhb field food fungal disease fusarium fusarium culmorum fusarium graminearum fusarium head blight gene genetic mapping genomic genomic regions graminearum head head blight head blight resistance heritability inoculation integrated control interval interval mapping losses mapping microsatellite molecular mapping mycotoxin pcr pcr marker penetration qtl qtl analysis quality resistance resistant scab scab resistance selection severity spring spring wheat ssr ssr marker triticum aestivum variance yield

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