GrainGenes Reference Report: BLT-26-1731
Reference
BLT-26-1731
Title
Real time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins
Journal
Biotechnology Letters
Year
2004
Volume
26
Pages
1731-1737
Author
Amoroso MG Longobardo L Capparelli R
Abstract
Developing seeds from Triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness
External Databases
Pubmed: 15604827
Keyword
[ Hide all but 1 of 15 ] cultivars flow cytometry flowering gene gene expression genes hardness kernel kernels plant proteins protein proteins seeds triticum aestivum wheat
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GrainGenes Reference Report: BLT-26-1731
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||