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GrainGenes Reference Report: EUP-139-167

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Reference	EUP-139-167
Title	DNA and morphological markers for a Russian wheat aphid resistance gene.
Journal	Euphytica
Year	2004
Volume	139
Pages	167-172
Author	Arzani A Peng JH Lapitan NLV
Abstract	The Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko), is a significant insect pest of wheat worldwide. Morphological and molecular markers associated with RWA resistance could be used to increase the accuracy and efficiency of selection of resistant germplasm and facilitate transfer to desirable wheat genotypes. The objective of this work was to identify microsatellite (SSR) markers linked to the RWA resistance gene (Dn4) and glume-colour gene (Rg2) using a population of F2-derived F3 families originating from a cross between a susceptible line (synthetic hexaploid-11) and a resistance cultivar (Halt). Two microsatellite markers Xgwm106 and Xgwm337 flanked Dn4 on the short arm of chromosome 1D at 5.9 and 9.2 cM, respectively. Two other microsatellite markers, Xpsp2999 and Xpsp3000, at the distal part of this chromosome arm are linked to Dn4 and to Rg2. The accuracy and efficiency of marker-assisted selection were calculated for homozygous Dn4Dn4 genotypes in the F2 generation. The gene Rg2 for red glume colour can also be used for marker-assisted selection of Dn4 gene individually and in combination with microsatellite markers. When used together, the closest markers Xgwm106 and Xgwm337, provide 100% accuracy and 75% efficiency. One hundred percent accuracy is also achieved when the morphological marker red glume is used in combination with either Xgwm106 or Xgwm337. Using these flanking markers, it may be possible to fix resistance to RWA in the first segregating generation of an F2 population without infestation with aphids
External Databases	DOI: 10.1007/s10681-004-2898-4
Keyword	[ Hide all but 1 of 18 ] colour genetic markers genotype genotypes germplasm glume glumes infestation insect pests marker-assisted selection markers microsatellite microsatellites molecular marker pest resistance plant pests population genetics resistance
Cited in	McIntosh RA et al. (2013) Catalogue of Gene Symbols for Wheat- 2013 Edition Catalogue of Gene Symbols for Wheat.

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