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GrainGenes Reference Report: EUP-139-59

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Reference
EUP-139-59
Title
Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai.
Journal
Euphytica
Year
2004
Volume
139
Pages
59-64
Author
[ Hide all but 1 of 8 ]
Zhang X
Zhou M
Ren L
Bai GH
Ma H
Scholten OE
Guo PG
Lu W
Abstract
Fusarium head blight (FHB) is a destructive disease of wheat worldwide. FHB resistance genes from Sumai 3 and its derivatives such as Ning 7840 have been well characterized through molecular mapping. In this study, resistance genes in Wangshuibai, a Chinese landrace with high and stable FHB resistance, were analyzed through molecular mapping. A population of 104 F SUB 2 -derived F SUB 7 recombinant inbred lines (RILs) was developed from the cross between resistant landrace Wangshuibai and susceptible variety Alondra's'. A total of 32 informative amplified fragment length polymorphism (AFLP) primer pairs ( Eco RI/ Mse I) amplified 410 AFLP markers segregating among the RILs. Among them, 250 markers were mapped in 23 linkage groups covering a genetic distance of 2,430 cM. In addition, 90 simple sequence repeat (SSR) markers were integrated into the AFLP map. Fifteen markers associated with three quantitative trait loci (QTL) for FHB resistance ( P <0.01) were located on two chromosomes. One QTL was mapped on 1B and two others were mapped on 3B. One QTL on 3BS showed a major effect and explained up to 23.8% of the phenotypic variation for type II FHB resistance
Keyword
amplified fragment length polymorphism
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