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GrainGenes Reference Report: BMI-17:92

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Reference	BMI-17:92
Title	Genomic insights from the first chromosome-scale assemblies of oat (Avena spp.) diploid species
Journal	BMC Biology
Year	2019
Volume	17
Author	Maughan PJ [ Show all 14 ]
Abstract	Background. Cultivated hexaploid oat (Common oat; Avena sativa) has held a significant place within the global crop community for centuries; although its cultivation has decreased over the past century, its nutritional benefits have garnered increased interest for human consumption. We report the development of fully annotated, chromosome-scale assemblies for the extant progenitor species of the As- and Cp-subgenomes, Avena atlantica and Avena eriantha respectively. The diploid Avena species serve as important genetic resources for improving common oat's adaptive and food quality characteristics. Results. The A. atlantica and A. eriantha genome assemblies span 3.69 and 3.78 Gb with an N50 of 513 and 535 Mb, respectively. Annotation of the genomes, using sequenced transcriptomes, identified ca. 50,000 gene models in each species-including 2965 resistance gene analogs across both species. Analysis of these assemblies classified much of each genome as repetitive sequence (ca. 83%), including species-specific, centromeric-specific, and telomeric-specific repeats. LTR retrotransposons make up most of the classified elements. Genome-wide syntenic comparisons with other members of the Pooideae revealed orthologous relationships, while comparisons with genetic maps from common oat clarified subgenome origins for each of the 21 hexaploid linkage groups. The utility of the diploid genomes was demonstrated by identifying putative candidate genes for flowering time (HD3A) and crown rust resistance (Pc91). We also investigate the phylogenetic relationships among other A- and C-genome Avena species. Conclusions. The genomes we report here are the first chromosome-scale assemblies for the tribe Poeae, subtribe Aveninae. Our analyses provide important insight into the evolution and complexity of common hexaploid oat, including subgenome origin, homoeologous relationships, and major intra- and intergenomic rearrangements. They also provide the annotation framework needed to accelerate gene discovery and plant breeding.
External Databases	https://doi.org/10.1186/s12915-019-0712-y
Germplasm	CC7277 (oat) BYU132 (oat)
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AE033855 AE033863 AE033864 AE033865 AE033868 AE033870 AE033871 AE033883 AE033885 AE033886 AE033887 AE033893 AE033899 AE033902 AE033912 AE033916 AE033936 AE033937 AE033951 AE033956 AE033965 AE033972 AE033978 AE033980 AE033981 AE033986 AE034004 AE034014 AE034023 AE034025 AE034036 AE034037 AE034041 AE034045 AE034061 AE034064 AE034066 AE034070 AE034072 AE034076 AE034082 AE034090 AE034105 AE034106 AE034107 AE034116 AE034120 AE034139 AE034166 AE034169 AE034170 AE034179 AE034188 AE034189 AE034190 AE034200 AE034219 AE034220 AE034226 AE034227 AE034230 AE034246 AE034253 AE034257 AE034266 AE034280 AE034283 AE034284 AE034288 AE034292 AE034300 AE034305 AE034309 AE034319 AE034320 AE034328 AE034333 AE034334 AE034336 AE034346 AE034347 AE034357 AE034360 AE034365 AE034366 AE034371 AE034372
Map Data	Oat-2019-A_atlantica-physical Oat-2019-A_eriantha-physical Oat-2019-A_atlantica-assembly Oat-2019-A_eriantha-assembly
Probe	AA000003 [ Show all 98057 ]
Sequence	AA049051 [ Show all 100205 ]

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