GrainGenes Reference Report: FDC-224-280
Reference
FDC-224-280
Title
Rapid quantitation of avenanthramides in oat-containing products by high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (HPLC-TQMS)
Journal
Food Chemistry
Year
2017
Volume
224
Pages
280-288
Author
[ Hide all but 1 of 9 ] Xie Z Mui T Sintara M Ou B Johnson J Chu Y O'shea M Kasturi P Chen Y
Abstract
Avenanthramides (AVNs) are a family of nitrogen-containing phenolic compounds produced in oat; AVN 2c, 2p, and 2f are the three major members. An LC-MS/MS method was developed, with the limit of detection (LOD) and the limit of quantitation (LOQ) being, respectively, 0.29ng/mL and 1.96ng/mL for AVN 2c, 0.24ng/mL and 0.60ng/mL for AVE 2p, and 0.42ng/mL and 2.2ng/mL for AVN 2f. The method was validated in oat-containing hot cereal and snack bar samples. The recovery of AVN 2c, 2p, and 2f from these two oat products was 95-113%, and the relative standard deviations ranged from 5% to 9%. This method was used to evaluate oat products and raw oat samples. The effects of location and variety on AVN composition were investigated. The method presented here provides a novel and rapid tool to quantitate the abundance of AVN 2c, 2p, and 2f in oat-containing products.
External Databases
https://doi.org/10.1016/j.foodchem.2016.12.079
Germplasm
AAC Nicolas (oat) AAC Oaklin (oat) AAC Roskens (oat)
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: FDC-224-280
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||