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GrainGenes Reference Report: TAG-133-1081

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Reference	TAG-133-1081
Title	Identification of molecular markers for the Pc39 gene conferring resistance to crown rust in oat
Journal	Theoretical and Applied Genetics
Year	2020
Volume	133
Pages	1081-1094
Author	Sowa S Paczos-Grzeda E
Abstract	The aim of this study was the identification of molecular markers for the Pc39 gene in cultivated oat (Avena sativa L.). Pc39 is a major race-specific crown rust resistance gene originally found in an Israeli accession of the wild hexaploid Avena sterilis. The effectiveness of this gene in Europe has decreased in recent years, but is still relatively high and breeding programs would benefit from the availability of molecular markers to aid in its mapping and deployment. The complexity of the oat genome poses a significant obstacle to genetic research. No oat rust resistance genes have yet been cloned, and even the number of relevant molecular markers is very limited. Here, genotyping of a segregating population derived from a cross 'Celer' (Pc39)/STH9210 (susceptible) was conducted using RAPD- and SRAP-PCR-based methods, as well as microarray-based DArT(tm) and next-generation sequencing DArTseq(tm) techniques. Markers associated with Pc39 were placed on the hexaploid oat consensus linkage group Mrg11 at 3.7-6.7 cM. Six new PCR-based markers were developed to allow identification of the resistant Pc39 allele. These tightly linked markers will be useful in marker-assisted selection, with the closest, SCAR_3456624, being within 0.37 cM of Pc39. The newly developed markers could find applications in the fine mapping or positional cloning of this gene. Moreover, easy-to-use PCR-based markers linked to Pc39 could facilitate the utilization of this gene in oat breeding programs, especially as a component of crown rust resistance gene pyramids.
External Databases	https://doi-org/10.1007/s00122-020-03533-z
Locus	[ Hide all but 1 of 61 ] 3277488 3279713 3280216 3280390 3281055 3281687 3282296 3282361 3282501 3282614 3282662 3282919 3282933 3453998 3454137 3454210 3454213 3454271 3454282 3454302 3454329 3454357 3454540 3454695 3454823 3454825 3454851 3454981 3455021 3455125 3455136 3455252 3455287 3455626 3455639 3455731 3455743 3455780 3455782 3455834 3455982 3456213 3456248 3456529 3454401_sD3 3455997b 3456272_sD2 3456624_sD1 SCAR_3454401 SCAR_3456272 SCAR_3456624 SCAR_oPt_17172 SCAR_RAPD_H11 SCAR_SRAP_Me23+Em14 avgbs_246256.1.9 avgbs_88849 avgbs_88849.1.10 avgbs_cluster_32397.1.62 avgbs2_122557.1.35 oPt-17172 Pc39
Map Data	Oat-2020-Celx9210_Pc39

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