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GrainGenes Reference Report: CAM-7-577

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Reference	CAM-7-577
Title	Semi-specific PCR for the evaluation of diversity among cultivars of wheat and triticale
Journal	Cellular and Molecular Biology Letters
Year	2002
Volume	7
Pages	577-582
Author	Gawe M Wisniewska I Rafalski A
Abstract	The usefulness of a semispecific PCR system for the evaluation of genetic diversity among selected cultivars of wheat ( Triticum aestivum L.) and triticale (x Triticosecale Wittm.) was investigated. A comparison was made of the diversity among cultivars of both species as revealed by primers targetting the semi-conservative sequences of the intron-exon junction. The main purpose of the study was the selection of the most informative primers from among the exon targetting (ET) and intron targetting (IT) primers of 12 to 18 bases in length. For triticale, the most satisfactory results were obtained using ET and IT primers of 18 bases in length. In wheat, complex and polymorphic banding patterns were generated by primers belonging to both groups, regardless of their length
External Databases	Pubmed: 12378263
Keyword	[ Hide all but 1 of 14 ] acclimatization analytical methods biochemistry cultivar diversity exons genetic diversity genetic polymorphism introns nucleotide sequence plant breeding polymerase chain reaction selection triticale

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