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GrainGenes Reference Report: TAG-135-2725

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Reference	TAG-135-2725
Title	Identification and characterization of the novel leaf rust resistance gene Lr81 in wheat
Journal	Theoretical and Applied Genetics
Year	2022
Volume	135
Pages	2725-2734
Author	Xu X [ Show all 8 ]
Abstract	Key message. The novel, leaf rust seedling resistance gene, Lr81, was identified in a Croatian breeding line and mapped to a genomic region of less than 100 Kb on chromosome 2AS. Abstract. Leaf rust, caused by Puccinia triticina, is the most common and widespread rust disease in wheat. Races of Puccinia triticina evolve rapidly in the southern Great Plains of the USA, and leaf rust resistance genes often lose effectiveness shortly after deployment in wheat production. PI 470121, a wheat breeding line developed by the University of Zagreb in Croatia, showed high resistance to Puccinia triticina races collected from Oklahoma, suggesting that PI 470121 could be a leaf rust resistance source for the southern Great Plains of the USA. Genetic analysis based on an F2 population and F2:3 families derived from the cross PI 470121 x Stardust indicated that PI 470121 carries a dominant seedling resistance gene, designated as Lr81. Linkage mapping delimited Lr81 to a genomic region of 96,148 bp flanked by newly developed KASP markers Xstars-KASP320 and Xstars-KASP323 on the short arm of chromosome 2A, spanning 67,030,206-67,132,354 bp in the Chinese Spring reference assembly (IWGSC RefSeq v1.0). Deletion bin mapping assigned Lr81 to the terminal bin 2AS-0.78-1.00. Allelism tests indicated that Lr81 is a distinctive leaf rust resistance locus with the physical order Lr65-Lr17-Lr81. Marker-assisted selection based on a set of markers closely linked to leaf rust resistance genes in PI 470121 and Stardust enabled identification of a recombinant inbred line RIL92 carrying Lr81 only. Lr81 is a valuable leaf rust resistance source that can be rapidly introgressed into locally adapted cultivars using KASP markers Xstars-KASP320 and Xstars-KASP323.
External Databases	https://doi.org/10.1007/s00122-022-04145-5
Germplasm	PI 470121
Probe	[ Hide all but 1 of 29 ] Xstars-KASP292 Xstars-KASP307 Xstars-KASP310 Xstars-KASP298 Xstars-KASP301 Xstars-KASP317 Xstars-KASP320 Xstars-KASP323 Xstars-KASP332 Xstars-KASP335 Xstars-KASP338 Xstars-KASP341 Xstars-KASP344 Xstars-KASP350 Xstars10 Xstars12 Xstars16 Xstars19 Xstars21 Xstars23 Xstars27 Xstars484 Xstars485 Xstars486 Xstars491 Xstars493 Xstars1002 Xstars1003 Xstars1013

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