GrainGenes Reference Report: TAG-139:14
Reference
TAG-139:14
Title
Novel resistance loci against Pyrenophora teres f. teres map to chromosomes 3H and 6H of barley
Journal
Theoretical and Applied Genetics
Year
2026
Volume
139
Author
[ Hide all but 1 of 13 ] Haydaroglu US Karakaya A Acar AC Celik Ojuz A Clare SJ Cetin Ozkan G Ergun N Aydogan S Karaoglu C Olmez F Tanyolac MB Sahin ES Brueggeman RS
Abstract
Pyrenophora teres f. teres, the fungus responsible for the barley disease, net-form net blotch (NFNB), leads to considerable yield and quality reductions. This research involved collecting phenotypic and genotypic data from a barley doubled haploid (DH) mapping population consisting of 277 lines, which were exposed to the highly virulent Ptt isolate GPS18. The DH lines were derived via anther culture from second-generation hybrids of a cross between the disease-resistant barley cultivar Avcı 2002 ('A') and the susceptible cultivar Bulbul 89 ('B'). Anther pretreatment with 1.0 M mannitol resulted in a statistically superior response compared to 0.7 M mannitol in the F2 progeny of the A x B cross. The highest callus induction rate was 37.6% in the 'Br_Ind' medium, and the highest green plant formation rate was 24.7% in the 'PD_Reg' medium. The use of sequencing-based diversity array technology (DArT-seq) identified 9170 SNP markers, which facilitated the creation of a linkage map spanning 1682.97 cM, with an average density of 1.49 markers/cM. Quantitative trait loci (QTL) analysis identified three QTL associated with Ptt resistance located on chromosomes 3H, 4H, and 6H. All three can be considered novel with the 3H QTL mapping in between Rpt1 and QRptta3, the 4H QTL maps to a distinct region of Rpt7, and the 6H QTL maps in between the Qns-6H.3 and SFNB-6H-33.74 loci. The SNPs associated with disease resistance identified within these QTL offer a foundation for developing DNA-based tests for resistance.
External Databases
https://doi.org/10.1007/s00122-025-05119-z
QTL
Ptt_3H Ptt_4H Ptt_6H
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-139:14
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||