GrainGenes Reference Report: TAG-129-831
Reference
TAG-129-831
Title
Isolation and fine mapping of Rps6: an intermediate host resistance gene in barley to wheat stripe rust
Journal
Theoretical and Applied Genetics
Year
2016
Volume
129
Pages
831-843
Author
[ Hide all but 1 of 6 ] Dawson AM Ferguson JN Gardiner M Green P Hubbard A Moscou MJ
Abstract
A plant may be considered a nonhost of a pathogen if all known genotypes of a plant species are resistant to all known isolates of a pathogen species. However, if a small number of genotypes are susceptible to some known isolates of a pathogen species this plant may be considered an intermediate host. Barley (Hordeum vulgare) is an intermediate host for Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust. We wanted to understand the genetic architecture underlying resistance to Pst and to determine whether any overlap exists with resistance to the host pathogen, Puccinia striiformis f. sp. hordei (Psh). We mapped Pst resistance to chromosome 7H and show that host and intermediate host resistance is genetically uncoupled. Therefore, we designate this resistance locus Rps6. We used phenotypic and genotypic selection on F2:3 families to isolate Rps6 and fine mapped the locus to a 0.1 cM region. Anchoring of the Rps6 locus to the barley physical map placed the region on a single fingerprinted contig spanning a physical region of 267 kb. Efforts are now underway to sequence the minimal tiling path and to delimit the physical region harboring Rps6. This will facilitate additional marker development and permit identification of candidate genes in the region.
External Databases
https://doi.org/10.1007/s00122-015-2659-x
QTL
QRpsh_7H.7 QRpsh_7H.6 QRpsh_7H.5
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-129-831
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||