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GrainGenes Reference Report: CCM-72-100

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Reference	CCM-72-100
Title	Characterization of low molecular weight glutenin subunits in durum wheat by reversed-phase high-performance liquid chromatography and N-terminal sequencing
Journal	Cereal Chemistry
Year	1995
Volume	72
Pages	100-104
Author	Masci S Lew E Lafiandra D Porceddu E Kasarda D
Abstract	Low molecular weight glutenin subunits (LMW-GS) from a residue preparation were fractionated by reversed-phase high-performance liquid chromatography (RP-HPLC) of reduced subunits from two biotypes of the Italian durum wheat cultivar Lira that differ at the Gli-B1/Glu*-B3 loci, such that Lira 42 has the LMW-1 pattern (associated with poor quality) and Lira 45 has the LMW-2 pattern (associated with good quality) of LMW-GS N-terminal amino acid sequencing was conducted on the major protein fractions (peaks) that differed chromatographically between the two types The fractions included four types of LMW-GS on the basis of their N-terminal sequences, which differed in their proportions among the various fractions These N-terminal sequences corresponded to the Ser-His-Ile- (LMW-s) type, the Met-Glu-Thr- (LMW-m) type, the alpha-(gliadin) type, and the gamma-(gliadin) type The LMW-s type was strongly predominant in the Lira 45 preparation and predominant in the Lira 42 preparation The LMW-m type was next most important in the Lira 45 preparations, but in the Lira 42 preparations, the percentage of alpha-type sequence was higher than the LMW-m type Both alpha-type and gamma-type sequences were stronger in Lira 42 than they were in Lira 45. Results indicated that differences in quality between the Lira biotypes may derive from: 1) the relative predominance in Lira 45 glutenins of the LMW-s type and the LMW-m type subunits, probably acting as glutenin polymer chain extenders; 2) the high proportion of the alpha-type and gamma-type glutenin subunits in the Lira 42 glutenins, probably included in the glutenin polymers because they have an odd number of cysteines and. act as glutenin polymer chain terminators; 3) the absolute amount of LMW glutenins, which is greater for LMW-2; or 4) some combination of these factors.
Keyword	[ Hide all but 1 of 8 ] amino acid sequence biotypes food-analysis genotype glutenin molecular-weight triticale wheat

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