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GrainGenes Reference Report: CCM-79-279

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Reference	CCM-79-279
Title	Environmental conditions during wheat grain development alter temporal regulation of major gluten protein genes
Journal	Cereal Chemistry
Year	2002
Volume	79
Pages	279-285
Author	Altenbach SB Kothari KM Lieu D
Abstract	Summary: The accumulation of transcripts for the major gluten storage proteins was assessed during grain development in wheat plants (Triticum aestivum L. 'Butte 86') grown under seven controlled environments where temperature, water, and fertilizer conditions were varied after anthesis. Transcripts within the alpha-, gamma- and omega-gliadin, low molecular weight glutenin subunit (LMW-GS), and high molecular weight glutenin subunit (HMW-GS) gene families followed similar patterns of accumulation throughout grain development under each environmental regimen. Under moderate daytime temperatures in plants that were well watered and fertilized, transcripts from all gene families were detectable by eight days postanthesis (8 DPA), were present at high levels until approximately equal to 34 DPA, and disappeared between 36 and 38 DPA. Under high temperature regimens, transcripts from all gene families appeared slightly earlier, but the time frame of accumulation was shorter. The presence or absence of postanthesis fertilizer did not alter the temporal regulation of the gluten genes under the moderate temperature regimen. However, under the high daytime temperature regimen, transcripts disappeared slightly earlier in kernels from plants that did not receive postanthesis fertilizer. Reverse transcriptase polymerase chain reaction (RT-PCR) using primer pairs for specific HMW-GS and LMW-GS transcripts revealed that individual genes within each family exhibited identical patterns of temporal regulation under different environmental conditions
Keyword	[ Hide all but 1 of 50 ] 20 allelic variation alpha anthesis baking quality chain environment environmental family fertilizer gamma gene gene family gliadin gluten glutenin glutenin subunit grain development high molecular weight high molecular weight glutenin high molecular weight glutenin subunit high temperature high-level high-temperature hmw-gs kernel lmw gs low molecular weight glutenin messenger rnas molecular weight omega-gliadin pattern polymerase polymerase chain reaction primer protein protein gene red winter wheat regulation reverse transcriptase storage storage protein subunit temperature transcript triticum triticum aestivum water weight glutenin subunit wheat grain

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