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GrainGenes Reference Report: CRS-33-595

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Reference	CRS-33-595
Title	Barley yellow dwarf virus resistance in a wheat X wheatgrass population
Journal	Crop Science
Year	1993
Volume	33
Pages	595-599
Author	Goulart L Mackenzie S Ohm H Lister R
Abstract	The barley yellow dwarf viruses (BYDVs) constitute an important group of plant viruses of cultivated cereal crops in which only tolerance and/or low levels of resistance are known High levels of resistance, which we here define as low concentrations of virus in plant tissues, have been discovered in Thinopyrum spp (formerly Agropyron) In this study we have characterized such resistance in BC2 and BC, populations, derived by crossing cultivated wheat (Triticum aestivum L emend Thell. with Th. ponticum (Podp.) Barkw. & D.R. Dewey Agropyron elongatum [Syn (Host) P. Beauv.] and backcrossing to wheat. The objectives were to evaluate the levels of resistance genetically transferable to wheat lines using both enzyme-linked immunosorbent assay (ELISA) and cDNA dot-blot hybridization for the detection of low levels of virus in plant tissues. Uniformity in results using both techniques depends on procedural uniformity, but a high correlation (0.93) between ELISA values and densitometry scans was obtained. A range in resistance was observed among the BC2 and BC3 populations from highly resistant to moderately resistant to susceptible. Lines classified as resistant had densitometry absorbance values of 0.25 to 0.60 and ELISA values from .03 to 0.30. The low values for both ELISA and dot-blot assays indicate that significant resistance can be maintained in more advanced generations. Such low virus levels also suggest that inhibition of virus replication or cell to cell movement may occur in resistant Thinopyrum spp.
Keyword	[ Hide all but 1 of 12 ] backcrossing barley-yellow-dwarf-luteovirus detection disease resistance dna hybridization elisa elymus elongatus elymus hispidus genetic resistance plant breeding population triticum aestivum

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