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GrainGenes Reference Report: CRS-33-902

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Reference	CRS-33-902
Title	Molecular cytogenetic analysis in wheat
Journal	Crop Science
Year	1993
Volume	33
Pages	902-908
Author	Gill B
Abstract	Two technical innovations led to major advances in the molecular cytogenetic analyses of the wheat genome The first innovation, the application of banding techniques, permitted distinction between heterochromatic and euchromatic regions and led to individual identification of metaphase chromosomes The second innovation, the development of in situ hybridization protocols, permitted molecular analysis of chromosome structure, and physical localization of specific DNA sequences in individual chromosomes The applications of banding and in situ hybridization techniques in cytogenetics, plant breeding, gene mapping, and in assaying alien chromatin introgression are briefly discussed A connection is made to the pioneering contributions of E.R. Sears, without which, rapid progress in the molecular cytogenetic analysis of wheat would not have occurred.
Keyword	[ Hide all but 1 of 16 ] assays chromosome-analysis chromosome-banding cytogenetics dna hybridization euchromatin gene-mapping genetic analysis heterochromatin identification in situ hybridization innovations introgression literature-reviews molecular genetics triticum aestivum

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