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GrainGenes Reference Report: CRS-40-1749

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Reference	CRS-40-1749
Title	Wheat transformation using cyanamide as a new selective agent
Journal	Crop Science
Year	2000
Volume	40
Pages	1749-1754
Author	Weeks JT Koshiyama KY Maier-Greiner U Schaeffner T Anderson OD
Abstract	Summary: There is a general need for additional selectable marker genes for plant transformation. Only a few have been reported in wheat (Triticum aestivum L.) transformation experiments, some of which are under patent restriction or have other disadvantages. A new selectable marker gene was identified which can be used to select resistant callus in tissue culture and regenerate transgenic wheat plants. A gene from the soil fungus Myrothecium verrucaria (Albertini & Schwein.) Ditmar:Fr., coding for the enzyme cyanamide hydratase which converts cyanamide into urea, was previously described. In our wheat transformation experiments, the gene conferred resistance to cyanamide at a tissue culture level and therefore cyanamide could be used to select for transformants. At the whole plant level, progeny of transformed wheat plants showed resistance to cyanamide, whereas sensitive plants expressed a lethal necrosis and yellowing when cyanamide was applied. This gene has several potential advantages when compared with other selectable marker genes. Transformed wheat plants can be selected at the tissue culture level and may be able to convert cyanamide into a useful nitrogen compound (fertilizer). The selectable marker gene could be introduced with other genes for value-added traits in wheat and might also be applicable in other transformation systems
Keyword	[ Hide all but 1 of 30 ] callus culture enzyme fertile transgenic wheat fertilizer fungus gene herbicide-resistance marker marker genes microprojectile bombardment necrosis nitrogen plant regeneration plant transformation progeny resistance resistant selectable marker selectable marker gene soil tissue tissue culture transformation transgenic transgenic wheat triticum aestivum urea wheat plants wheat transformation

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