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GrainGenes Reference Report: CRS-41-1584

Reference	CRS-41-1584
Title	A Microsatellite marker for tagging Dn2, a wheat gene conferring resistance to the Russian wheat aphid
Journal	Crop Science
Year	2001
Volume	41
Pages	1584-1589
Author	Miller CA Altinkut A Lapitan NLV
Abstract	Summary: The Russian wheat aphid (RWA), Diuraphis noxia Mordvilko, is an economically important pest of wheat (Triticum aestivum L.). An effective means to control the RWA is through the use of resistant cultivars. While a phenotype-based selection has been useful for selection of resistant plants, it has inherent limitations. Screening can only be done during cool months of the year, and symptom expression is influenced by the environment. Pyramiding of two or more RWA resistance genes is also difficult because of the presence of only one aphid biotype in the USA at present. This study was conducted to develop a DNA marker that is tightly linked to Dn2, and to test the effectiveness of the marker as a tag for Dn2 among a limited number of cultivars tested. We report mapping of five microsatellite markers linked to Dn2. The closest marker was Xgwm437 at 2.8 cM, and it distinguished lines containing Dn2 from eight susceptible cultivars and seven resistant cultivars carrying other RWA resistance genes. Xgwm437 should be effective for marker-assisted selection of Dn2-containing plants and for combining Dn2 with other resistance genes in a gene pyramiding program
Keyword	[ Hide all but 1 of 49 ] aphid biotype bread wheat bulked segregant analysis conferring resistance crop cultivar cultivars diuraphis noxia dna dna marker environment expression gene gene pyramiding gene tagging genes genetic mapping genetic markers genetic resistance inheritance limitation line linkage map mapping marker marker-assisted selection microsatellite microsatellite marker monosomic analysis pest pest resistance program pyramiding rapd resistance resistance gene resistant russian wheat aphid rwa screening selection susceptibility symptoms triticum triticum aestivum triticum tauschii usa

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