Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: CRS-42-2088

[Submit comment/correction]

Reference	CRS-42-2088
Title	Modified rapid DNA extraction protocol for high throughput microsatellite analysis in wheat
Journal	Crop Science
Year	2002
Volume	42
Pages	2088-2091
Author	Hill-Ambroz KL Brown-Guedira GL Fellers JP
Abstract	Summary: New technology is allowing marker-assisted selection to fulfill the promise of increasing efficiency of cultivar development. However, these techniques depend upon the ability to extract DNA from large populations of plants. The objective of this project was to develop a high-throughput DNA extraction procedure without the need for greenhouse space or growing wheat (Triticum aestivum L.) plants. A sodium hydroxide rapid DNA extraction was modified for a 96-well format to reduce costs. Seeds were germinated in 8-well tissue culture plates, and 4-d-old seedling tissue was used to extract DNA by means of sodium hydroxide methodology. Approximately 1 microgram of genomic DNA per 10 mg of tissue was isolated at a cost of about $0.10. The DNA quality was verified by amplification of microsatellite markers. Results were consistent with either fresh or stored tissue extracts. This technique allows one person to extract nearly 1000 storage-stable DNA samples daily, while keeping costs at a minimum
Keyword	[ Hide all but 1 of 23 ] amplification bread wheat crop cultivar culture dna dna extraction extract extraction genomic genomic dna locus map methodology microsatellite project protocol quality selection sodium tissue triticum triticum aestivum

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.