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GrainGenes Reference Report: GNM-36-397

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Reference	GNM-36-397
Title	Variability among members of the Hor-2 multigene family
Journal	Genome
Year	1993
Volume	36
Pages	397-403
Author	Kanazin V Ananiev E Blake T
Abstract	The hordeins comprise the major prolamin storage proteins of barley Two major and one minor gene families encode these alcohol-soluble proteins The Hor-2 gene family encoding the B-hordeins has been estimated to contain 15-30 copies. Although several genes encoding B-hordeins have been cloned and sequenced, little is known about the mechanisms responsible for the generation of the enormous genetic variability at this locus. Polymerase chain reaction sequence amplification provided a simple technique that permitted the amplification of the Hor-2 gene family members from the genomes of several barley genotypes. Sequence analysis of clones permitted the identification of a region within the Hor-2 structural gene that appears to undergo recombinational and slippage-like gene conversion events. In this report we describe variability of the B-hordein genes, possible mechanisms responsible for it, and implications this may have on the evolution of prolamin-encoding gene families.
Keyword	[ Hide all but 1 of 13 ] gene-conversion genetic polymorphism hor-2 gene hordein hordeum hordeum vulgare multigene-families polymerase chain reaction recombination restriction mapping restriction-endonuclease-analysis structural genes wild-plants

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