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GrainGenes Reference Report: GNM-38-153

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Reference	GNM-38-153
Title	Pulsed-field gel analysis of 5S and satellite DNA in barley
Journal	Genome
Year	1995
Volume	38
Pages	153-157
Author	Roder M Sorrells M Tanksley S
Abstract	Pulsed-field gel electrophoresis was used to study the variability of clustered tandemly repeated sequences in barley Twelve spring barley cultivars were investigated with a heterologous 5S DNA probe and the 118 base pair barley satellite DNA probe HVT01 On a per fragment basis, the 5S probe was 5 times and the barley satellite probe 6.7 times more variable than single- or low-copy RFLP markers, demonstrating their usefulness for cultivar distinction.
Keyword	[ Hide all but 1 of 10 ] cultivar dna fingerprinting dna hybridization dna probes genes hordeum vulgare pulsed-field-electrophoresis ribosomal dna ribosomal-rna satellite-dna

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