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GrainGenes Reference Report: GNM-42-821

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Reference	GNM-42-821
Title	Molecular genetic mapping of three X-linked avirulence genes, vH6, vH9 and vH13, in the Hessian fly
Journal	Genome
Year	1999
Volume	42
Pages	821-828
Author	Schulte SJ Rider SD Hatchett JH Stuart JJ
Abstract	Summary: Three X-linked avirulence genes, vH6, and vH9, and vH13 in the Hessian fly, Mayetiola destructor, confer avirulence to Hessian fly resistance genes H6, H9, and H13 in wheat. We used a combination of two- and three-point crosses to determine the order of these genes with respect to each other, the white eye mutation and three X-linked molecular markers, G15-1, 020, and 021, developed from genomic lambda clones, lambdaG15-1, lambda020, and lambda021. The gene order was determined to be vH9-vH6-G15-1-w-vH13-020-021. In situ hybridization of lambdaG15-1, lambda020, and lambda021, on the polytene chromosomes of the Hessian fly salivary gland established their orientation on Hessian fly chromosome X1. Based on the size of the Hessian fly genome, and the genetic distances between markers, the relationship of physical to genetic distance was estimated at no more than 300 kb/cM along Hessian fly chromosome X1, suggesting that map-based cloning of these avirulence genes will be feasible
External Databases	Pubmed: 10584305
Keyword	[ Hide all but 1 of 23 ] chromosome cloning dna hybridization gene genetic distance genetic mapping genetic markers genetic resistance genome hybridization linkage mapping mayetiola destructor molecular genetics molecular mapping molecular marker mutation pest resistance phenotypes resistance triticum aestivum virulence x chromosome

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