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GrainGenes Reference Report: GNM-43-945

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Reference	GNM-43-945
Title	Organization of highly repeated sequences in surface-spread pachytene chromosomes of rye
Journal	Genome
Year	2000
Volume	43
Pages	945-948
Author	Cunado N Barrios J Santos JL
Abstract	Summary: A method of preparing two-dimensional surface spreads of plant synaptonemal complexes (SCs) associated with fluorescence in situ hybridization (FISH) has been applied to analyze the location and organization of five different highly repeated DNA sequences in rye. Our observations indicate that, depending on the type of sequence, the chromatin displays different types of organization. Telomeric sequences were seen tightly associated with the SC while other repetitive DNA sequences were found to form loops that are associated with SCs only at their bases. On the contrary, the FISH signal of a centromeric satellite had a granular appearance, reflecting that the hybridization occurs only with parts of the chromatin loops
Keyword	[ Hide all but 1 of 30 ] appearance bases canada chromatin dna dna sequence fish fluorescence fluorescence in situ hybridization heterochromatin hybridization in situ in situ hybridization localization meiosis method nuclei organization pachytene pachytene chromosome repeated dna repetitive dna repetitive dna sequence rye sequence signal spread surface synaptonemal complex telomere

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