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GrainGenes Reference Report: GNM-44-266

Reference	GNM-44-266
Title	Identification of Bilby, a diverged centromeric Ty1-copia retrotransposon family from cereal rye (Secale cereale L.)
Journal	Genome
Year	2001
Volume	44
Pages	266-274
Author	Francki MG
Abstract	Summary: A diminutive rye chromosome (midget) in wheat was used as a model system to isolate a highly reiterated centromeric sequence from a rye chromosome. Fluorescence in situ hybridization (FISH) shows this sequence localized within all rye centromeres and no signal was detected on wheat chromosomes. DNA sequencing of the repetitive element has revealed the presence of some catalytic domains and signature motifs typical of retrotransposon genes and has been called the Bilby family, representing a diverged family of retrotransposon-like elements. Extensive DNA data-base searching revealed some sequence similarity to centromeric retrotransposons from wheat, barley, and centromeric repetitive sequences from rice. Very low levels of signal were observed when Bilby was used as a probe against barley, and no signal was detected with rice DNA during Southern hybridization. The abundance of Bilby in rye indicates that this family may have diverged from other distantly related centromeric retrotransposons or incorporated in the centromere but rapidly evolved in rye during speciation. The isolation of a rye retrotransposon also allowed the analysis of centromeric breakpoints in wheat-rye translocation lines. A quantitative analysis shows that the breakpoint in 1DS.1RL and 1DL.1RS and recombinant lines containing proximal rye chromatin have a portion of the rye centromere that may contribute to the normal function of the centromeric region
Keyword	[ Hide all but 1 of 46 ] 1rs barley canada catalytic domains centromere chromatin chromosomal localization chromosome database dna dna sequencing domain element family fish fluorescence fluorescence in situ hybridization gene genome heterogeneity hybridization in situ in situ hybridization induction isolate isolation midget chromosome model organization quantitative analysis repetitive dma sequences repetitive element repetitive sequence retrotransposon rice rye secale cereale sequence sequence similarities signal situ hybridization speciation translocation translocation lines wheat chromosomes wheat-rye translocation

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