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GrainGenes Reference Report: GNM-44-936

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Reference	GNM-44-936
Title	Low responsiveness of six-rowed genotypes to androgenesis in barley does not have a pleiotropic basis
Journal	Genome
Year	2001
Volume	44
Pages	936-940
Author	Castillo AM Cistue L Romagosa I Valles M Valles MP
Abstract	A heterozygous mutant for the two- and six-rowed character was isolated in the barley cultivar Igri through application of sodium azide to isolated microspore cultures and posterior regeneration. Six-rowed and two-rowed homozygotic plants were subsequently identified in the self-pollinated M2 progenies of the original heterozygous M1. Detailed molecular markers confirmed the isogenic nature of this recovered mutant and the original cultivar Igri. A comparative study of the anther culture response of this six-rowed induced mutant vs. diploid 'Igri' was performed to assess whether the two- or six-rowed gene influences anther culture response in barley through a pleiotropic effect or via linkage disequilibrium. No significant differences for any of the recorded variables throughout the in vitro regeneration process were detected between the 'Igri' six-rowed mutant and any of their two-rowed isogenic lines. This suggests that row-type association with anther culture response in barley cultivars is due to the effect of a tight linkage with other genes directly responsible for androgenic response
Keyword	[ Hide all but 1 of 48 ] androgenesis anther anther culture anther culture response barley barley cultivars callus formation canada character chromosome cultivar culture differentiation diploid diploidy disequilibrium gene genotype heterozygosity hordeum vulgare in vitro in vitro regeneration induced mutant isogenic lines line linkage linkage disequilibrium marker markers microspore microspore culture molecular molecular marker mutant mutation pleiotropy pollination polymorphism progeny regeneration response selfing six-rowed sodium sodium azide two-rowed vitro vrs1 locus

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