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GrainGenes Reference Report: GNM-44-1129

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Reference	GNM-44-1129
Title	Molecular characterization of a Thinopyrum intermedium Group 2 chromosome (2Ai-2) conferring resistance to barley yellow dwarf virus
Journal	Genome
Year	2001
Volume	44
Pages	1129-1135
Author	Zhang ZY Xin ZY Larkin PJ
Abstract	Summary: The whea--Thinopyrum intermedium addition lines Z1 and Z2 carry 21 pairs of wheat chromosomes and one pair of Th. intermedium chromosomes (2Ai-2) conferring resistance to barley yellow dwarf virus (BYDV). GISH results using the genomic DNA of Pseudoroegneria strigosa (S genome) as the probe indicated that the 2Ai-2 chromosome in Z1 and Z2 is an S-J intercalary translocation. Most of the 2Ai-2 chromosome belongs to the S genome, except for about one third in the middle region of the long arm that belongs to the J genome. The results of detailed RFLP analyses confirmed that the 2Ai-2 chromosome is extensively homoeologous to wheat group 2 chromosomes. Some new RFLP markers specific to the 2Ai-2 chromosome were identified. A RAPD marker, OP-R16(340), specific to the 2Ai-2 chromosome, was screened. We converted the RAPD marker into a sequence-characterized amplified region (SCAR) marker (designated SC-R16). The study establishes the basis for selecting translocation lines with small segments of the 2Ai-2 chromosome and localizing the BYDV resistance gene when introgressed into a wheat background. Total number of records in report: 92
Keyword	[ Hide all but 1 of 48 ] addition addition line addition lines barley barley yellow dwarf barley yellow dwarf virus barley yellow dwarf virus (bydv) belongs bydv canada chromosome conferring resistance dna dwarf dwarf virus gene genome genomic dna genomic in situ hybridization (gish) gish homoeologous group 2 in situ hybridization intermedium long arm marker molecular characterization partial amphiploids ponticum rapd region resistance resistance gene rflp rflp marker scar segment strigosa thinopyrum thinopyrum intermedium tolerance translocation translocation line translocation lines virus wheat chromosomes winter wheat yellow dwarf yellow dwarf virus

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