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GrainGenes Reference Report: GNM-45-646

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Reference	GNM-45-646
Title	Assessment of genomic diversity among wheat genotypes as determined by simple sequence repeats
Journal	Genome
Year	2002
Volume	45
Pages	646-651
Author	Ahmad M
Abstract	Summary: Simple sequence repeats (SSRs) have been used to examine the genomic diversity of wheat (Triticum aestivum L.) germplasm. Thirteen wheat genotypes of diverse origin were analyzed with 43 selected SSRs to provide uniform and maximum genome coverage. A total of 156 allelic variants were detected at 43 SSR loci, ranging from two to eight per locus with an average of 3.6. The polymorphic information content (PIC) values of the loci ranged from 0.10 (Xgwm264) to 0.89 (Xgwm471 and Xgwm577). Genetic similarities calculated from SSR data ranged from 30.1 ('Era' and 'Klasic') to 90.1 ('Neepawa' and 'Thatcher') between genotypes. UPGMA analysis based on genetic distance estimates produced three loose groupings that were generally consistent with available pedigree information. Cultivars 'Neepawa' and 'Thatcher' are closely related. Their genetic relationship was confirmed by the facts that they share a common ancestor and are clustered together. There were two different 'Era' genotypes, one used in the 'Otane' pedigree and one used in this study. None of the other genotypes had a close common ancestor indicating any close genetic relationships. Principal coordinate analysis also confirmed this pattern of genetic diversity. A wide range of genomic diversity was observed among all the genotypes, proving them to be prime candidates for selective breeding for specific traits and broadening the genetic base
External Databases	Pubmed: 12175067
Keyword	[ Hide all but 1 of 42 ] 30 assessment base bread wheat breeding canada cluster analysis distance diversity dna marker genetic distance genetic diversity genetic relationship genetic relationships genetic similarity genetic similarity estimates genetic-distance genetic-relationships genome germplasm heterosis information maximum microsatellite origin parentage pattern pedigree pic polymorphic polymorphism principal coordinate analysis relationships sequence similarities trait triticum triticum aestivum upgma variant wheat genotypes wide

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