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GrainGenes Reference Report: HER-86-738

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Reference	HER-86-738
Title	Evolutionary dynamics and chromosomal distribution of repetitive sequences on chromosomes of Aegilops speltoides revealed by genomic in situ hybridization
Journal	Heredity
Year	2001
Volume	86
Pages	738-742
Author	Belyayev A Raskina O Nevo E
Abstract	Summary: Simultaneous genomic in situ hybridization (GISH) with probe preannealing was used to detect the relationship between chromosomal position and sequence conservation on Aegilops speltoides var. aucheri chromosomes. DNA of Secale sylvestre, Hordeum spontaneum, Festuca pratensis, Semiarundinaria fastuosa, Arundo donax and Zea mays that represent several main groups of Poaceae were used as probes. Different GISH-banding patterns that characterize diverse evolutionary trajectories in the repetitive DNA fraction and correlate with evolutionary distance between tested species were observed. Fast-evolving sequences were detected in subterminal telomeric and subtelomeric heterochromatic regions, whereas sequences in pericentromeric regions showed high levels of conservation. GISH experiments revealed extensive conservation in NOR regions on chromosomes 1 and 6 which, in fact, appears to be a complicated mix of rDNA clusters and heterochromatin blocks of different nucleotide composition
External Databases	Pubmed: 11595054
Keyword	[ Hide all but 1 of 46 ] aegilops aegilops speltoides chromosome cluster composition conservation distance dna dna sequences dynamics evolutionary festuca festuca pratensis fraction genomic in situ hybridization gish heterochromatic regions heterochromatin high-level hordeum hordeum spontaneum hybridization in situ in situ hybridization mays nor nucleotide pattern pericentromeric regions poaceae position pratensis probes rdna region repetitive dna repetitive dna sequences repetitive sequence secale sequence situ hybridization speltoides spontaneum triticeae zea zea mays

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