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GrainGenes Reference Report: JEB-50-1627

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Reference	JEB-50-1627
Title	Cloning of a cDNA encoding the thaumatin-like protein of winter rye (Secale cereale L. Musketeer) and its functional characterization
Journal	Journal of Experimental Botany
Year	1999
Volume	50
Pages	1627-1628
Author	Chan YW Tung WL Griffith M Chow KC
Abstract	Summary: A cDNA clone, WRTLP2, encoding an open reading frame of 173 amino acids, was recovered from a cDNA library of winter rye (Secale cereale L. Musketeer). The amino acid sequence deduced from the cloned cDNA exhibits very high sequence similarity (70-95%) with those of extracellular and low molecular weight thaumatin-like proteins of other cereals. It was possible to overexpress this isolated cDNA in Escherichia coli and it was found that the encoded protein of this clone exhibited antifungal activities against several fungal strains
Keyword	[ Hide all but 1 of 14 ] amino acid amino acid sequence antifungal properties cloning complementary dna escherichia coli gene expression molecular weight open reading frame pathogenesis related proteins plant proteins secale cereale weight winter

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